21 Loading data
Ryabhatta
Seurat objects either processed through Natian or through any other method can be used in Ryabhatta. The only requirement is that the data must be processed with a version of Seurat compatabile with Ryabhatta. In the current version of Ryabhatta (version 3), users need to process the data using Seurat R version 4.0 or later. The data may be stored in any of the following formats
21.1 Load .rds/.RDS file
Single seurat objects can be stored as .RDS or .rds data format. These datasets can be loaded to Ryabhatta, but require a name. The name entered in the input box
The loaded Seurat object from the .RDS object will be linked with this label name.
21.2 Load .RData file
Another native format for storing R data is .Rdata. The main difference between .RDS and .RData object is that .RData can contain multiple Seurat object and other data instead of just one Seurat object as contained within the .RDS format.
Names provided in the input box will not be used for the objects within the .RData. Instead, the original names provided for the Seurat objects within the .Rdata will be linked with the data sets.
.RDatafiles generated in one version of R may not be compatible with another. This may affect recognition of the data in Ryabhatta and lead to the app crashing.